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Sterilisation temperature simultaneously. EN285 states that, for a typical industrial autoclave, all heat penetration thermocouples distributed within the load, should reach sterilising temperature within 30 seconds. Any delay indicates either a failure to remove air or a failure to adequately heat the load and, as such, the. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F). These temperatures (and other high temperatures) 830 must be maintained for a minimal time to kill microorganisms. Temperature Measurement in Freezers, Autoclaves, and Air. Also remove all the air from inside a sensor if the sensor and cable are in the autoclave. When steam is added, the vacuum inside the sensor. Durability is a big problem with autoclave air temperature measurement. The extra pressure in an autoclave means that water boils at a temperature higher than its normal boiling point—roughly 20°C hotter—so it holds and carries more heat and kills microbes more effectively. Whether it’s a small tabletop autoclave or a room-sized bulk autoclave, all autoclaves operate on similar principles that they share with a common kitchen pressure cooker — the door is locked to form a sealed chamber, and all air within the chamber is replaced by steam. The steam is then pressurized to reach the desired sterilization temperature and time, before exhausting the steam and allowing the goods to be removed. Air temperature inside autoclave.